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Sangon Biotech spin column fungal total rna purification kit
Spin Column Fungal Total Rna Purification Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spin+column+fungal+total+rna+purification+kit/column+dna+extraction+gel+kit+sanprep/pm42073377-87-6-13
Average 86 stars, based on 1 article reviews
spin column fungal total rna purification kit - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Purification:

Article Title: Carbonic anhydrases contribute to mitochondrial function, conidial development, and pathogenicity of Magnaporthe oryzae
Article Snippet: .. Total RNA was extracted from 7-day-old fungal colonies grown on PDA medium using the Spin Column Fungal Total RNA Purification Kit (Sangon Biotech). .. For cDNA synthesis, All-in-One 5X RT MasterMix (Applied Biological Materials Inc.) was used to reverse transcribe 2 μg of total RNA from each strain. qRT-PCR was subsequently performed using the CFX96 Optical Reaction Module (BIO-RAD, Singapore) and BlasTaqTM 2×qPCR MasterMix (Applied Biological Materials Inc.).

Article Title: FgNdk1 Promotes Effector Secretion to Scavenge ROS During Fusarium graminearum Infection
Article Snippet: .. Total RNA was extracted using a spin column fungal total RNA purification kit (Sangon Biotech, B518659‐0100). ..

Article Title: Carbonic anhydrases contribute to mitochondrial function, conidial development, and pathogenicity of Magnaporthe oryzae .
Article Snippet: .. The strains were inoculated in the above medium, inverted in an incubator at 28°C for 7 days. qRT-PCR Total RNA was extracted from 7-day-old fungal colonies grown on PDA medium using the Spin Column Fungal Total RNA Purification Kit (Sangon Biotech). .. For cDNA synthe sis, All-in-One 5X RT MasterMix (Applied Biological Materials Inc.) was used to reverse transcribe 2 μg of total RNA from each strain. qRT-PCR was subsequently performed using the CFX96 Optical Reaction Module (BIO-RAD, Singapore) and BlasTaqTM 2×qPCR MasterMix (Applied Biological Materials Inc.).

Article Title: Effects of Rootstock and Exogenous Plant Growth Regulators on Volatile Aroma Profiles and Terpenoid-Mediated Defense in Table Grape Fruit.
Article Snippet: .. Total RNA was extracted using the Spin Column Fungal Total RNA Purification Kit (Sangon Biotech, Shanghai, China) and subsequently reverse https://doi.org/10.3390/life16040567 transcribed into cDNA. ..

Article Title: BdAreA regulates nitrogen metabolism, stress response, and virulence in Botryosphaeria dothidea
Article Snippet: .. Total RNA was extracted from mycelia collected at different time points using the Spin Column Fungal Total RNA Purification Kit (Sangon Biotech, Shanghai). .. Subsequently, 1 μg of total RNA was reverse-transcribed into cDNA using the HiScript II 1st-Strand cDNA Synthesis Kit (with gDNA removal function, Vazyme).

Article Title: Quercetin 2,3-dioxygenase genes VmQDO1 and VmQDO2 of Valsa mali utilizes flavonoid to produce two key toxins for pathogenesis.
Article Snippet: Apple Valsa canker, caused by the necrotrophic fungus Valsa mali, is a devastating disease in East Asia.. V. mali employs cell wall-degrading enzymes and toxins to induce cortical necrosis, while also degrading host flavonoids, key plant defense metabolites, to facilitate infection and toxin production.. Here, we identified two quercetin 2,3dioxygenase genes, VmQDO1 and VmQDO2, in V. mali, which are significantly upregulated during infection and in response to quercetin and rutin.

Article Title: Antifungal activity of volatile organic compounds produced by Bacillus subtilis GB519 against blast pathogen Magnaporthe oryzae in rice
Article Snippet: .. Total RNA was extracted from the mycelia using a spin column fungal total RNA purification Kit (Sangon Biotech (Shanghai) Co., Ltd). .. First-strand cDNA was synthesized using a ToloScript All-in-one RT EasyMix (TOLO Biotech (Shanghai) Co., Ltd).

Article Title: Effects of Rootstock and Exogenous Plant Growth Regulators on Volatile Aroma Profiles and Terpenoid-Mediated Defense in Table Grape Fruit
Article Snippet: .. Total RNA was extracted using the Spin Column Fungal Total RNA Purification Kit (Sangon Biotech, Shanghai, China) and subsequently reverse transcribed into cDNA. ..

Quantitative RT-PCR:

Article Title: Carbonic anhydrases contribute to mitochondrial function, conidial development, and pathogenicity of Magnaporthe oryzae .
Article Snippet: .. The strains were inoculated in the above medium, inverted in an incubator at 28°C for 7 days. qRT-PCR Total RNA was extracted from 7-day-old fungal colonies grown on PDA medium using the Spin Column Fungal Total RNA Purification Kit (Sangon Biotech). .. For cDNA synthe sis, All-in-One 5X RT MasterMix (Applied Biological Materials Inc.) was used to reverse transcribe 2 μg of total RNA from each strain. qRT-PCR was subsequently performed using the CFX96 Optical Reaction Module (BIO-RAD, Singapore) and BlasTaqTM 2×qPCR MasterMix (Applied Biological Materials Inc.).

RNA Extraction:

Article Title: Quercetin 2,3-dioxygenase genes VmQDO1 and VmQDO2 of Valsa mali utilizes flavonoid to produce two key toxins for pathogenesis.
Article Snippet: Apple Valsa canker, caused by the necrotrophic fungus Valsa mali, is a devastating disease in East Asia.. V. mali employs cell wall-degrading enzymes and toxins to induce cortical necrosis, while also degrading host flavonoids, key plant defense metabolites, to facilitate infection and toxin production.. Here, we identified two quercetin 2,3dioxygenase genes, VmQDO1 and VmQDO2, in V. mali, which are significantly upregulated during infection and in response to quercetin and rutin.

Isolation:

Article Title: Quercetin 2,3-dioxygenase genes VmQDO1 and VmQDO2 of Valsa mali utilizes flavonoid to produce two key toxins for pathogenesis.
Article Snippet: Apple Valsa canker, caused by the necrotrophic fungus Valsa mali, is a devastating disease in East Asia.. V. mali employs cell wall-degrading enzymes and toxins to induce cortical necrosis, while also degrading host flavonoids, key plant defense metabolites, to facilitate infection and toxin production.. Here, we identified two quercetin 2,3dioxygenase genes, VmQDO1 and VmQDO2, in V. mali, which are significantly upregulated during infection and in response to quercetin and rutin.

Reverse Transcription:

Article Title: Effects of Rootstock and Exogenous Plant Growth Regulators on Volatile Aroma Profiles and Terpenoid-Mediated Defense in Table Grape Fruit
Article Snippet: .. Total RNA was extracted using the Spin Column Fungal Total RNA Purification Kit (Sangon Biotech, Shanghai, China) and subsequently reverse transcribed into cDNA. ..



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Sangon Biotech spin column fungal total rna purification kit
Spin Column Fungal Total Rna Purification Kit, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/spin+column+fungal+total+rna+purification+kit/column+dna+extraction+gel+kit+sanprep/pm42073377-87-6-13
Average 86 stars, based on 1 article reviews
spin column fungal total rna purification kit - by Bioz Stars, 2026-10
86/100 stars
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